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zp2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology zp2
    Zp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+zp2/ZP2+Antibody/pm41819103-1093-10-11
    Average 93 stars, based on 27 article reviews
    zp2 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: The Effect of Normal Follicular Fluid on the Differentiation of PCOS Ovarian Stem Cells into Oocyte-Like Cells
    Article Snippet: .. Afterward, the incubation of primary antibodies was performed with anti-NANOG (rabbit antihuman, 1:200; Bio Legend), anti-OCT4 (rabbit antihuman, 1:200; S Bio Legend), anti-ZP2, and anti-ZP3 (mouse monoclonal, 1:100; Santa Cruz Biotechnology) antibodies at 4°C overnight. .. Later, cells were washed three times with PBS and incubated with fluorescein isothiocyanate conjugated (FITC) goat anti-rabbit or rabbit anti-mouse antibodies (Sc2012; Santa Cruz Biotechnology, Inc.) diluted at 1:500 in PBS 1X at RT for 1 h. In the final step, after three washes with PBS 1X, cell nuclei were stained with 4′, 6-diamidino-2-phenylindole (DAPI).

    Article Title: Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell
    Article Snippet: .. Afterwards, the blocking 192 solution (1% BSA,1x PBS) was used to block non-specific binding of the antibodies for 30-45 193 min, and incubation was done with anti-vimentin (rabbit antihuman at a ratio of 1:200; Santa 194 Cruz), anti-OCT4 (rabbit antihuman, 1:200; Santa Cruz), anti-NANOG (rabbit antihuman, 1:200; 195 Santa Cruz), anti-ZP2 and anti-ZP3 (mouse monoclonal,1:100; Santa Cruz) antibody at 4°C 196 overnight. .. Once the cells were rinsed with PBS three times, they were incubated by rabbit anti197 mouse or goat anti-rabbit FITC-conjugated antibodies (Sc2012; Santa Cruz Biotechnology, Inc., 198 Dallas, TX, USA) diluted at a ratio of 1:500 in PBS-1x at room temperature for one hour.

    Article Title: Utilizing Calcium Alginate for the Assessment of Bone Morphogenetic Protein 15 Induction Effect on the Differentiation of Mesenchymal Stem Cell Derived from Human Follicular Fluid to Oocyte-Like Structure
    Article Snippet: .. In the next step, the beads were rinsed three times with PBS containing washing buffer, and then incubation was done with anti-OCT4 (rabbit antihuman, 1:100; Bio Legend), anti-NANOG (rabbit antihuman, 1:100; Bio Legend), and anti-ZP2 and anti-ZP3 (mouse monoclonal, 1:50; Santa Cruz) antibodies at 4°C overnight. .. Again, the beads were washed three times with PBS, transferred to a 1.5-mL conical tube, and incubated with fluorescein isothiocyanate-conjugated goat anti-rabbit or rabbit anti-mouse antibodies (Sc2012; Santa Cruz Biotechnology, Inc.) diluted at 1:200 in PBS at 37°C for 2 h. Then, 0.4-μL/mL DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) in PBS was used for staining cellular nuclei for 20 min.

    Article Title: Oocyte Arrested at Metaphase II Stage Were Derived From Human Pluripotent Stem Cells in Vitro
    Article Snippet: After electrophoresis, proteins were transferred to a PVDF membrane (LC2002, Invitrogen) by semi-dry electrophoretic transfer (Bio-Rad) for 45 min at 15V. .. The membrane was blocked with 5% non-fat milk for 1 h at room temperature, and then incubated with the primary antibodies, including anti-OCT4 (1:500, SC-5279, Santa Cruz), anti-BMP15 (1:1000, ab108413, Abcam), anti-SCP3 (1:1000, ab181746, Abcam), anti-ZP2 (1:500, 32894, Santa Cruz), anti-ZP3 (1:300, SC398359, Santa Cruz), and anti-GAPDH (1:2000, KM9002, Sungene Biotech), for 2 h. After washing three times with TBS-T buffer (20 mM Tris/HCl pH 7.6, 137 mM NaCl, 0.1% Tween 20), the membrane was incubated with an HRP-conjugated secondary antibody (1:3000, A0258, Beyotime) at 37°C for 1 h. After washing in TBST (Tris-buffered saline, 0.1% Tween 20) for three times at room temperature, the membrane was incubated in the enhanced chemiluminescent substrate (#32106, Pierce) for 1 min and detected with a Chemiluminescent Imaging System (ZY058176, Tanon-4200). ..

    Blocking Assay:

    Article Title: Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell
    Article Snippet: .. Afterwards, the blocking 192 solution (1% BSA,1x PBS) was used to block non-specific binding of the antibodies for 30-45 193 min, and incubation was done with anti-vimentin (rabbit antihuman at a ratio of 1:200; Santa 194 Cruz), anti-OCT4 (rabbit antihuman, 1:200; Santa Cruz), anti-NANOG (rabbit antihuman, 1:200; 195 Santa Cruz), anti-ZP2 and anti-ZP3 (mouse monoclonal,1:100; Santa Cruz) antibody at 4°C 196 overnight. .. Once the cells were rinsed with PBS three times, they were incubated by rabbit anti197 mouse or goat anti-rabbit FITC-conjugated antibodies (Sc2012; Santa Cruz Biotechnology, Inc., 198 Dallas, TX, USA) diluted at a ratio of 1:500 in PBS-1x at room temperature for one hour.

    Binding Assay:

    Article Title: Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell
    Article Snippet: .. Afterwards, the blocking 192 solution (1% BSA,1x PBS) was used to block non-specific binding of the antibodies for 30-45 193 min, and incubation was done with anti-vimentin (rabbit antihuman at a ratio of 1:200; Santa 194 Cruz), anti-OCT4 (rabbit antihuman, 1:200; Santa Cruz), anti-NANOG (rabbit antihuman, 1:200; 195 Santa Cruz), anti-ZP2 and anti-ZP3 (mouse monoclonal,1:100; Santa Cruz) antibody at 4°C 196 overnight. .. Once the cells were rinsed with PBS three times, they were incubated by rabbit anti197 mouse or goat anti-rabbit FITC-conjugated antibodies (Sc2012; Santa Cruz Biotechnology, Inc., 198 Dallas, TX, USA) diluted at a ratio of 1:500 in PBS-1x at room temperature for one hour.

    Membrane:

    Article Title: Oocyte Arrested at Metaphase II Stage Were Derived From Human Pluripotent Stem Cells in Vitro
    Article Snippet: After electrophoresis, proteins were transferred to a PVDF membrane (LC2002, Invitrogen) by semi-dry electrophoretic transfer (Bio-Rad) for 45 min at 15V. .. The membrane was blocked with 5% non-fat milk for 1 h at room temperature, and then incubated with the primary antibodies, including anti-OCT4 (1:500, SC-5279, Santa Cruz), anti-BMP15 (1:1000, ab108413, Abcam), anti-SCP3 (1:1000, ab181746, Abcam), anti-ZP2 (1:500, 32894, Santa Cruz), anti-ZP3 (1:300, SC398359, Santa Cruz), and anti-GAPDH (1:2000, KM9002, Sungene Biotech), for 2 h. After washing three times with TBS-T buffer (20 mM Tris/HCl pH 7.6, 137 mM NaCl, 0.1% Tween 20), the membrane was incubated with an HRP-conjugated secondary antibody (1:3000, A0258, Beyotime) at 37°C for 1 h. After washing in TBST (Tris-buffered saline, 0.1% Tween 20) for three times at room temperature, the membrane was incubated in the enhanced chemiluminescent substrate (#32106, Pierce) for 1 min and detected with a Chemiluminescent Imaging System (ZY058176, Tanon-4200). ..

    Saline:

    Article Title: Oocyte Arrested at Metaphase II Stage Were Derived From Human Pluripotent Stem Cells in Vitro
    Article Snippet: After electrophoresis, proteins were transferred to a PVDF membrane (LC2002, Invitrogen) by semi-dry electrophoretic transfer (Bio-Rad) for 45 min at 15V. .. The membrane was blocked with 5% non-fat milk for 1 h at room temperature, and then incubated with the primary antibodies, including anti-OCT4 (1:500, SC-5279, Santa Cruz), anti-BMP15 (1:1000, ab108413, Abcam), anti-SCP3 (1:1000, ab181746, Abcam), anti-ZP2 (1:500, 32894, Santa Cruz), anti-ZP3 (1:300, SC398359, Santa Cruz), and anti-GAPDH (1:2000, KM9002, Sungene Biotech), for 2 h. After washing three times with TBS-T buffer (20 mM Tris/HCl pH 7.6, 137 mM NaCl, 0.1% Tween 20), the membrane was incubated with an HRP-conjugated secondary antibody (1:3000, A0258, Beyotime) at 37°C for 1 h. After washing in TBST (Tris-buffered saline, 0.1% Tween 20) for three times at room temperature, the membrane was incubated in the enhanced chemiluminescent substrate (#32106, Pierce) for 1 min and detected with a Chemiluminescent Imaging System (ZY058176, Tanon-4200). ..

    Imaging:

    Article Title: Oocyte Arrested at Metaphase II Stage Were Derived From Human Pluripotent Stem Cells in Vitro
    Article Snippet: After electrophoresis, proteins were transferred to a PVDF membrane (LC2002, Invitrogen) by semi-dry electrophoretic transfer (Bio-Rad) for 45 min at 15V. .. The membrane was blocked with 5% non-fat milk for 1 h at room temperature, and then incubated with the primary antibodies, including anti-OCT4 (1:500, SC-5279, Santa Cruz), anti-BMP15 (1:1000, ab108413, Abcam), anti-SCP3 (1:1000, ab181746, Abcam), anti-ZP2 (1:500, 32894, Santa Cruz), anti-ZP3 (1:300, SC398359, Santa Cruz), and anti-GAPDH (1:2000, KM9002, Sungene Biotech), for 2 h. After washing three times with TBS-T buffer (20 mM Tris/HCl pH 7.6, 137 mM NaCl, 0.1% Tween 20), the membrane was incubated with an HRP-conjugated secondary antibody (1:3000, A0258, Beyotime) at 37°C for 1 h. After washing in TBST (Tris-buffered saline, 0.1% Tween 20) for three times at room temperature, the membrane was incubated in the enhanced chemiluminescent substrate (#32106, Pierce) for 1 min and detected with a Chemiluminescent Imaging System (ZY058176, Tanon-4200). ..



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    Journal: Poultry Science

    Article Title: Dietary supplementation with selenium yeast mitigates diquat-induced oxidative damage in oviductal magnum of hens

    doi: 10.1016/j.psj.2025.105882

    Figure Lengend Snippet: The effect of SeY on the reproductive performance of the oviduct magnum of hens under the action of DQ. A. Immunostaining of Wnt4 ( n = 3). Scale bar: 100 μm. B-C. Relative mRNA expression of ZP2 and Wnt4 in hens’ magnum tissue of each group ( n = 3). D. Western blot of the ZP2 and Wnt4 proteins in hens’ magnum tissue of each group. E-F. Relative expression levels of ZP2 and Wnt4 proteins in hens’ magnum tissue of each group ( n = 3). Different letters indicate significant differences ( P < 0.05).

    Article Snippet: After blocking, membranes were incubated overnight at 4°C with primary antibodies (Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), ZP2 (PB0958, Boster, Wuhan, China), Wnt4 (A00879, Boster, Wuhan, China)) and β-actin (BM0627, Boster, Wuhan, China) as a control.

    Techniques: Immunostaining, Expressing, Western Blot